Prevalence, antimicrobial resistance, and biofilm formation of Klebsiella pneumoniae isolated from human and cows

Klebsiella pneumoniae (K. pneumoniae) is an important multidrug-resistant (MDR) and biofilm producing pathogen that causes diverse infections in human animals. This study aimed to evaluate the prevalence, antimicrobial resistance, forming ability of K. isolates from diseased humans sputum urine samples mastitic cows milk Zagazig city, Sharkia Governorate, Egypt. A total 100 animal were collected examined for presence species. The recovered identified by conventional bacteriological procedures confirmed traditional PCR at genus species levels using gyrA, 16S-23S ITS pehX genes, respectively. subjected testing their susceptibility formation ability. results revealed 10 out 50 positive (20%); 28% (7/25) 12% (3/25) samples. Additionally, prevalence rate was 4% (2/50). assays 12 as pneumoniae. showed had high resistance rates ampicillin ampicillin/clavulanate (100% each) with multiple antibiotic (MAR) index 0.083, followed azithromycin, cefepime, trimethoprim/sulphamethoxazole (91.7% each). Meanwhile, these sensitivity chloramphenicol (83.3%) MAR 0.014. Furthermore, 91.7% able produce biofilm, where 8.3% a non-biofilm producer. Out 11 isolates, 6 (54.5%), 3 (27.3%) 2 (18.2%) moderate, strong weak producers, present emphasized MDR Therefore, more attentions should be taken against antimicrobials usage providing new antibiofilm approaches biofilms.